RIPK1 Knockout cell line(HT1080)

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location： Home > Products > KO Cell Lines > RIPK1 Knockout cell line(HT1080)

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RIPK1 Knockout cell line(HT1080) Order

RIPK1 Knockout cell line(HT1080)
Catalog#
YKO-HJ060
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RIPK1 Knockout cell line(HT1080)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RIPK1 Knockout cell line (HT1080)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
RIPK1
Gene ID
8737
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_D9WE(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript RIPK1-201)
Validation: PCR+Sanger Sequencing
RIPK1 Knockout cell line(HT1080) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HT-1080		 STR Info (Cell bank)
Cell Line:HT-1080
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 12141214
D7S820 910910
D16S539 912912
VWA 14191419
TH01 6666
AMEL XYXY
TPOX 8888
CSF1PO 12121212
D12S391 2022
FGA 2225
D2S1338 2526
D21S11 282930.0
D18S51 1218
D8S1179 1314
D3S1358 1616
D6S1043 1218
PENTAE 515
D19S433 13.215
PENTAD 912
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RIPK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-1080 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RIPK1 gene knockout RIPK1 Knockout cell line (HT1080) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RIPK1 Knockout cell line(HT1080)
RIPK1 Knockout cell line(HT1080)
Catalog#
YKO-HJ060
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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